Microbiological laboratories and procedures are designed to handle four different levels of etiologic agents. Materials which pose the least risk are designated Biosafety Level 1 and those posing the greatest risk, Biosafety Level 4. A combination of laboratory practices, techniques, safety equipment, and design features characterize each level. Only Biosafety Level 1 & 2 agents are currently used at Radford University.
Any Primary Investigator performing work at Biosafety Level 2 or higher is required to review and amended the Radford University Biosafety Manual (PDF) for their laboratory and or specific agent.
1.0 Biosafety Level 1
At level 1, practices, safety equipment, and facilities are appropriate for work that is performed with strains of viable microorganisms not known to cause disease in healthy adult humans.
Standard Microbiological Practices
Special Practices
Containment Equipment
Laboratory Facilities
2.0 Biosafety Level 2
This class of laboratory is suitable for work involving agents of moderate potential hazard to personnel and the public. In addition to the characteristics of a Biosafety Level 1 facility, (a) laboratory personnel have specific training in handling pathogenic agents and are directed by competent scientists, (b) access to the laboratory is limited whenever work is being conducted, and (c) when some procedures are conducted in which infectious aerosols are created, they will be performed in biological safety cabinets or other physical containment equipment.
Standard Microbiological Practices
Special Practices
Containment Equipment
Laboratory Facilities